Seed Biotechnology Lab group at CEMB

Research laboratory

Seed Biotechnology

The lab improves and commercialises economically important crops — cotton, corn, sugarcane, potato and soybean — engineering insect, herbicide and virus resistance and exploring plant metabolic engineering.

Research with field impact

From molecular insight to seed in the farmer’s hand.

Research spans transgenic trait engineering in cotton, maize, sugarcane, soybean and canola; RNA interference and CRISPR-Cas9 for biotic stress tolerance; virus-free seed potato production; and an emerging programme in plant metabolic engineering and synthetic biology to understand secondary metabolite pathways such as gossypol and steroidal glycoalkaloids. The lab is also a government-designated facility for commercial GMO detection and DNA fingerprinting services.

Transgenic crop traitsRNAi & genome editingSeed technology & GMO testing
9
approved cotton varieties
13
current research scholars
5
active funded projects
10+
years of virus-free seed potato delivery

Research programme

Four routes to stronger crop systems.

The group connects molecular design, controlled validation, field evaluation and regulatory translation in one integrated programme.

  1. 01

    Insect & herbicide resistant crops

    Engineered Bt and herbicide-tolerant traits commercialised in cotton, corn and sugarcane, including CEMB's approved double- and triple-gene cotton varieties.

  2. 02

    RNAi & CRISPR biopesticides

    RNA interference and CRISPR-Cas9 approaches to breed biotic stress-tolerant crops and dsRNA-based biopesticides.

  3. 03

    Transgenic maize & legumes

    Local Bt and herbicide-tolerant maize hybrids, plus insect- and herbicide-resistant soybean and canola under development.

  4. 04

    Seed technology & GMO testing

    Virus-free seed potato production, synthetic seed protocols, and designated commercial DNA/RNA/protein-based GMO detection services.

Translation & outcomes

Biotechnology built to leave the laboratory.

Commercial varieties, regulatory testing, field-ready crop lines and scalable propagation systems.

01

Four double-gene and five triple-gene cotton varieties approved by the Punjab Seed Council for general cultivation

02

Designated by national regulatory authorities for commercial DNA-, RNA- and protein-based GMO detection and crop-variety fingerprinting

03

Developed insect- and herbicide-resistant maize lines for hybrid breeding under Pakistan Biosafety Rules 2005

04

Developed insect- and herbicide-resistant sugarcane lines with promising sugar recovery

05

Established reproducible, cost-effective tissue culture and transformation protocols for sugarcane, maize, soybean and canola

06

Developed Fusarium resistance in highly susceptible Gladiolus cultivars

07

Established protocols for mass production of pre-basic virus-free seed potato, marketed by M/s AGB Seeds Pvt Limited for over 10 years

08

Developed synthetic seed production protocols for carrot, cucumber and potato

09

Conducted insect-pest bioassays and field evaluation of in-house Bt and Mycorrhiza-based biopesticides for cotton, tomato, cauliflower, okra and rice

Research group

Faculty leading the programme.

Expertise across plant transformation, genome editing, crop protection, molecular breeding and regulatory science.

Research students

The questions shaping tomorrow’s crops.

7 PhD scholars6 MPhil scholars

PhD research scholars 7

Noman Nazik

Noman Nazik

Development of resistant tomato lines against Tomato brown rugose fruit virus (ToBRFV) through genome editing

Amina Amin

Amina Amin

Expression of hybrid Bt SN-19 gene and dsRNA-Sec-23 in tomato against lepidopterans

Muniba Shahid

Muniba Shahid

dsRNA-based control of cotton bollworms

Misbah Saleem

Misbah Saleem

Deciphering molecular mechanisms underlying gossypol-mediated whitefly tolerance in cotton plants

Jawad-ul-Hassan

Jawad-ul-Hassan

Development of zinc-biofortified potato lines via integrated breeding

Baraka Siddique

Baraka Siddique

Integrated management of fruit flies in peach orchards using RNAi-mediated gene silencing and biocontrol agents

Amna Amjad

Amna Amjad

Using CRISPR-Cas systems to develop stress-resilient maize lines

MPhil research scholars 6

Zain Ahmed Khan

Zain Ahmed Khan

Development of multi-herbicide-tolerant soybean to enable diversified weed management

Laiba Nezami

Laiba Nezami

Targeting the cell-wall degradation machinery of Phytophthora infestans using in-vitro-synthesised dsRNA for sustainable management of potato late blight

Tehreem Irfan

Tehreem Irfan

CRISPR/Cas9-mediated editing of the soybean eIF4E gene for resistance to Soybean mosaic virus

Kainat Sajid

Kainat Sajid

Production of chitosan nanoparticle-coated dsRNA targeting chitin synthase in thrips and whiteflies

Samia Sarwar

Samia Sarwar

CRISPR-mediated knockout of ZmHSBP2 and heat-inducible overexpression of HOP3 to enhance thermotolerance in maize

Ayesha Munir

Ayesha Munir

Spatiotemporal expression profiling of stacked insecticidal and herbicide-tolerance transgenes in five-gene cotton lines

Research funding

A sustained pipeline of applied innovation.

Competitive public funding and industry partnerships support projects from prototype development through commercial translation.

Active projects

Highly efficacious sucking- and chewing-insect-resistant cotton prototype for commercialisationHEC Technology Development Fund TDF-04-302 · 2025–2026 · PKR 9.3 million
Genetic basis of resistance in pink bollworm and weeds in three-gene cotton fields of PunjabPunjab Agricultural Research Board · 2026–2029 · PKR 21.95 million
A non-GM, eco-friendly dsRNA-based biopesticide to control cotton pink bollwormPakistan Academy of Sciences, PSDP Project 302 · 2025 · PKR 12.105 million
Whitefly-resistant cotton through CRISPR-Cas9 and rewiring carbon flux in the gossypol pathwayHEC NRPU Project 17008 · PKR 3.5 million
Development of tomato lines resistant to Tomato brown rugose fruit virusIndustry-supported project · M.Y. Genetiks, Antalya, Türkiye

Completed projects

Genetic improvement of sugarcane for herbicide and borer resistancePunjab Agricultural Research Board · 2010–2015 · PKR 21.149 million
Commercialisation of CLCuV-tolerant indigenous transgenic Bt and glyphosate-resistant cotton hybridsPunjab Agricultural Research Board · 2012–2017 · PKR 21.149 million
Transforming the CEMB-PARB biotech maize prototype into viable commercial productsHigher Education Commission · 2012–2017 · PKR 14 million
Transformation of sucrose isomerase genes in sugarcane for boosted sugar recoveryHigher Education Commission · 2018–2021 · PKR 3.9 million
Genetic improvement of potato against cold-induced sweeteningHigher Education Commission · 2018–2021 · PKR 8.192 million
Genetic improvement in maize lines for insect resistance and glufosinate tolerancePunjab Agricultural Research Board · 2019–2023 · PKR 10 million

Collaborate with the lab

Bring a crop challenge. Build the next field-ready solution.

Contact the group lead